SELECTIVE PLANE ILLUMINATION MICROSCOPE (SPIM) WITH AN OPEN- TOP CONFIGURATION

Tech ID: 24649 / UC Case 2015-006-0

Invention Novelty

This technology provides a novel SPIM microscope set up that allows the user to image living specimens and specimens prepared in various formats including multi-well tissue culture chambers, microfluidic devices, and high-throughput screening assay platforms.

Value Proposition

The challenges associated with current imaging techniques such as epifluorescence and confocal microscopy include slow imaging times and phototoxic damage to samples. SPIM (also known as light-sheet microscopy) is a faster imaging technology that reduces phototoxicity and photobleaching by using a single sheet of light to image specimens in a single plane. This technology typically generates a 3D image of an immobilized specimen by acquiring a series of optically sectioned images.

Technology Description

Existing SPIM microscopes require samples to be immobilized in a gel cylinder or in other ways that restrict sample preparation. The researchers at University of California, San Francisco have developed a novel SPIM microscope with an open-top geometry resembling a conventional inverted microscope, thus not requiring specialized specimen preparation. Their technology can acquire 3D images living specimens in regular petridishes and can image in a high throughput format involving 96-well plates or microfluidic chambers.

Application

  • Imaging of living specimens including mammalian cells, living organisms, and tissue explants
  • High-throughput imaging of 96-well plates
  • Micro-fluidic experiments

Looking for Partners

We are looking for partners to develop and commercialize this technology as a selective plane illumination microscope for conventionally mounted specimens.

Stage of Development

Prototype

Related Materials

Data Availability

Under NDA/CDA

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,620,415 04/14/2020 2015-006
United States Of America Issued Patent 10,139,608 11/27/2018 2015-006
 

Other Information

PCT Application WO/2016/054474

Contact

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Inventors

  • Huang, Bo
  • McGorty, Ryan J.

Other Information

Keywords

Selective Plane Illumination Microscopy, Light sheet microscopy, Live imaging, Microscopy, High throughput, Light microscopy

Categorized As