This technology provides a novel SPIM microscope set up that allows the user to image living specimens and specimens prepared in various formats including multi-well tissue culture chambers, microfluidic devices, and high-throughput screening assay platforms.
The challenges associated with current imaging techniques such as epifluorescence and confocal microscopy include slow imaging times and phototoxic damage to samples. SPIM (also known as light-sheet microscopy) is a faster imaging technology that reduces phototoxicity and photobleaching by using a single sheet of light to image specimens in a single plane. This technology typically generates a 3D image of an immobilized specimen by acquiring a series of optically sectioned images.
Existing SPIM microscopes require samples to be immobilized in a gel cylinder or in other ways that restrict sample preparation. The researchers at University of California, San Francisco have developed a novel SPIM microscope with an open-top geometry resembling a conventional inverted microscope, thus not requiring specialized specimen preparation. Their technology can acquire 3D images living specimens in regular petridishes and can image in a high throughput format involving 96-well plates or microfluidic chambers.
We are looking for partners to develop and commercialize this technology as a selective plane illumination microscope for conventionally mounted specimens.
|United States Of America||Issued Patent||10,620,415||04/14/2020||2015-006|
|United States Of America||Issued Patent||10,139,608||11/27/2018||2015-006|
Selective Plane Illumination Microscopy, Light sheet microscopy, Live imaging, Microscopy, High throughput, Light microscopy