Sequential Array Cytometry: Multi-Parameter Imaging with a Single Fluorescent Channel

Tech ID: 22309 / UC Case 2011-252-0

Summary

UCLA researchers in the Department of Bioengineering have developed a novel method for performing quantitative and dynamic single-cell biomarker measurements at a single fluorescent wavelength by utilizing parallel hydrodynamic cell trapping arrays.

Background

A personalized approach to medical diagnostics and treatment is required due to heterogeneity within the human population and within diseased tissues. To that end, functional assays at the single-cell level can contribute to uncovering heterogeneity and ultimately assist in improved treatment decisions based on the presence of outlier cells.

Single-cell fluorescent microscopy provides a high level of intracellular resolution and dynamics of molecular events. However, the process is slow and manual. Flow cytometry on the other hand, has a high throughput but provides no intracellular resolution or dynamics of molecular events. An automated fluorescent microscopy using a scanning microscope provides a higher throughput and intracellular resolution but there are increased costs and complexity due to the optical requirements.

Innovation

Researchers at UCLA have developed a novel sequential array cytometry method that utilizes hydrodynamic cell trapping arrays to simplify optical requirements associated with high throughput cell analysis. The device provides for cellular imaging with high intracellular resolution and high content screening while utilizing an extremely simplified optical setup. The limit on the number of independent parameters that can be measured is no longer dependent on the complexity of the optics as all stains can be observed with a single light-source, filter set and camera.

Applications

  • Cell imaging
  • Drug discovery
  • Coupling to wide field of view imaging techniques and/or automated liquid handling for personalized high content screening for testing drug efficacy with a patient's own cells

Advantages

  • High intracellular resolution
  • High throughput screening
  • Simplified optical requirements
  • Measures up to 60 independent parameters

State Of Development

Researchers have demonstrated the technology's principle and have developed key aspects of a prototype.

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 9,222,870 12/29/2015 2011-252
 

Contact

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Inventors

  • Di Carlo, Dino

Other Information

Keywords

cytometry, microfluidics, single-cell, immunocytochemistry, high content screening, cellomics, drug discovery, personalized medicine, point-of-care

Categorized As