Increasing exposure of humans and animals to environmental endocrine disruptors is of great concern. Therefore, there is need for rapid and effective detecting systems for endocrine disruptors in environmental and biological samples. However, it is problematic that the current detecting systems cannot detect new chemicals that can act like testosterone (and other androgenic) or antiandrogenic chemicals, because these chemicals can be structurally diverse. A researcher at the University of California, Davis developed a novel, cell-based bioassay that detects diverse compounds, known and new, that impact the androgen receptor signaling pathway.
This stably transfected recombinant human cell line responds to androgenic chemcials with the induction of luciferase gene expression in a time-, dose-, androgen receptor-, and chemical-specific manner.
Non-exclusive licenses are available for UC's property rights in this cell bioassay and patent rights in the luciferase reporter gene as it is utilized within the cell bioassay.
This cell bioassay also contains components owned by Promega Corporation. Licensees can acquire Promega permissions relevant to practicing this invention by executing a contract services agreement directly with Promega. UC can provide interested parties with a draft license agreement as well as a sample of the Promega contract services agreement.
This novel, cell-based bioassay allows rapid, inexpensive and broad-based screening for the identification and characterization of androgenic/antiandrogenic chemicals. It detects any compound that can impact the androgen receptor signaling pathway, directly or indirectly, and thus can be used to detect both known and new androgenic/antiandrogenic chemicals. This is something that is not possible using analytical instrumental or immunoassay methods, which can only detect known androgenic/antiandrogenic chemicals. The assay can also provide an estimate of the relative androgenic/antiandrogenic potency of a target/test chemical or an extract containing these chemicals.
testosterone, androgen, bioassay, environmental endocrine disruptor