New Collision-Induced Dissociation Cross Linker and Related Software Package for Fast and Accurate Mass Spectrometry Analysis of Proteins

Brief Description

Researchers at the University of California, Irvine have developed a new and novel mass spectrometry (MS) cleavable disuccinimidyl sulfoxide (DSSO) cross-linker for MS analysis of proteins. This DSSO cross-linker contains two symmetric collision-induced dissociation (CID) cleavable sites that allow for effective identification of DSSO-cross-linked peptides based on their distinct fragmentation patterns unique to cross-linking types such as interlink, intralink, and dead-ends.

A software package was also developed to be used with the DSSO cross linker. This software performs an integrated data analysis workflow for identifying DSSO-cross-linked peptides.

Advantages

This new cross-linker allows for the MS analysis of multi-subunit protein complexes. In addition, the accompanying software package allows for the fast and accurate identification of DSSO-cross-linked peptides via MS.

State Of Development

This cross-linker and its accompanying software have been successfully used for the structural characterization of yeast 20 S proteasome complex and also demonstrated is identifying and characterizing model peptides and proteins.

Patent Status