|United States Of America||Issued Patent||9,029,169||05/12/2015||2011-054|
SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is a tool widely used in biochemistry, cell and molecular biology and clinical diagnosis to separate proteins based on their size. However, SDS treatment has a significant impact on protein original structure and may decrease the normal level of biological activity of the protein. Protein renaturation or removal of SDS is necessary prior to immuno-probing or mass spec identification, particularly for Western blotting.
UC Berkeley researchers have developed a microfluidic device and assay which streamlines all steps needed to obtain protein renaturation, mobility and binding-based identity information in one continuous assay. The assays complete in minutes and are readily adaptable to a broad range of multistage assays and are highly sensitive and specific.