Protein Renaturation Microfluidic Devices

Tech ID: 21177 / UC Case 2011-054-0

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 9,029,169 05/12/2015 2011-054
 

Brief Description

SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is a tool widely used in biochemistry, cell and molecular biology and clinical diagnosis to separate proteins based on their size.  However, SDS treatment has a significant impact on protein original structure and may decrease the normal level of biological activity of the protein.  Protein renaturation or removal of SDS is necessary prior to immuno-probing or mass spec identification, particularly for Western blotting.

 

UC Berkeley researchers have developed a microfluidic device and assay which streamlines all steps needed to obtain protein renaturation, mobility and binding-based identity information in one continuous assay.  The assays complete in minutes and are readily adaptable to a broad range of multistage assays and are highly sensitive and specific.

    

Suggested uses

 

  • Rapid and automated protein renaturation, separation and identification
  • Multiplexed protein blotting
  • Validation and point of care diagnostic assays

 

Advantages

 

  • Rapid assays (requires less than 5 minutes) provide large time savings over conventional methods
  • Does not require the use of SDS for separation of the protein based on size
  • Does not require manual intervention and is less labor intensive
  • One cohesive device optimized for protein renaturation, separation, transfer, dilution and blotting steps
  • Simultaneous multi-analyte detection
  • Consumes low volumes of reagents

 

Publication

Membrane Assisted on-line Renaturation for Automated Microfluidic Lectin Blotting

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Inventors

  • Herr, Amy E.

Other Information

Keywords

microfluidic, protein, renaturation, SDS, lab on a chip, diagnostic, blot, western blot

Categorized As