Researchers at UC Berkeley have developed a synthetic polymer interface for the long-term self-renewal of human embryonic stem cells (hESCs) in defined media. Current culture systems for hESCs require the use of isolated animal derived extracellular matrix proteins or mouse embryonic feeder cells. The proposed use of a completely synthetic cell culture substrate avoids the problems associated with the variability of and the exposure to animal products. The hydrogel network coating is comprised of aminopropylmethacrylamide (APMAAm) monomer and N,N-methylenebis(acrylamide) (bis) crosslinker that was grafted to standard tissue culture polystyrene (TCPS) dishes via photoinitiated addition polymerization. Results for hESC proliferation and pluripotency markers were both qualitatively and quantitatively similar to cells cultured on MatrigelÔ -coated substrates.
|United States Of America||Published Application||US140051163||02/20/2014||2011-019|
Allows for the long-term self-renewal of hESCs in completely defined conditions.
Does not require attachment of peptides or proteins to promote cell attachment.
Is readibly scalable to clinical-scale cell expansion.
Free of complex, undefined culture materials.