UCLA investigators developed a multivalent targeting strategy to modify drug carriers and enhance its targetability, sensitivity, and overall efficiency. This method involves the modification of drug-loaded nanoparticles, and has potential uses for drug delivery, imaging and medical applications.
Ligand binding to the surface of drug carriers has been explored as a way of enhancing targetability and overall efficiency of drug delivery and imaging applications. Multivalent binding to imaging agents has been investigated and has been demonstrated to increase targeting over monovalent binding. However, multivalent ligand binding to enhance targeting of drug-loaded nanoparticles has not been investigated. Therefore, there is a need to investigate the effect of multivalent binding to drug-loaded nanoparticles.
Researchers at UCLA have identified a multivalent targeting strategy that increased the targeting efficiency and specificity of drug carriers toward targeted cells. This strategy involves spatially constraining ligands, so that the ligands are presented as clusters rather than single molecules. The method involves the modification of DNA loaded nanoparticles with the ligand clusters. This type of an approach has been shown to be more effective at targeting cancer cells with high expressions of integrin receptors and more effective at delivering genes compared to drug carriers with homogeneously distributed ligands. Because of the enhanced targeting and gene transfer efficiency, it is believed that this approach is ideal for the delivery of nucleic acid based drugs.
Inventors have demonstrated their strategy in vitro using RGD and DNA/PEI polyplexes.
|United States Of America||Issued Patent||8,263,133||09/11/2012||2009-233|
drug delivery, non-viral, multivalent targeting, drug carriers, imaging applications, nanoparticles, ligands