Brief description not available

Brief description not available

Brief description not available

Brief description not available

The invention describes a platform technology that increases MHC presentation of oncogene derived peptide neoantigens that do not normally occur in the cell. The platform has already been used to identify a method of increasing KRAS G12 D/V derived peptide presentation on MHC- I.

A novel method to engineer a class of chimeric receptors to detect defined extracellular signals and modulate specified cell responses such as transcription, recombination, or signaling.  

This invention is a novel method of controlling gene expression at genome scale using CRISPRi/a, which provides for highly specific and robust induction or repression of transcription.

BACKGROUND:  There is a general need for dynamically regulatable protein binding domains to control functional protein interactions in a variety of experimental and commercial applications. The majority of such systems that have been developed are based on the administration of chemical dimerizers which require the slow, irreversible diffusion into the cell of small molecules that target the dimer-interface site. An alternative method is the control of protein interaction in any suitable host cell or organisms by light. This invention relates to a light-regulatable protein-protein interaction system based upon phytochromes, a family of photoreceptors that enable plants to adapt to their prevailing light environment. TECHNOLOGY:   UCSF inventors have developed the first genetically encoded system for the fine spatial and temporal control of the localization and activity of proteins on sub-micrometer and sub-second scales. The system has further been optimized to be modular and easily switched to future arbitrary signaling pairs and localization tags.

UCSF scientists have developed a method to engineer a synthetic, feedback-regulated MAPK signaling pathway using scaffold-mediated feedback loops. This method can be used to systematically re-program MAPK signaling responses, allowing one to engineer and modify the MAPK signaling pathway to optimally control dynamic and complex behaviors in living cells. Many potential applications exist, including engineering of metabolic processes for optimal biofuel production.

Cells have long been sorted by various means including through electrokinetic sorting, differential uptake of chemicals, magnetic antibodies specific to the target cell surface, and flow-cytometry assays. A key limitation to these methods is that they are either not sufficiently specific to isolate dead cells from live cells or they render the sorted cells unusable for clinical applications. UC investigators have developed a cell sorting platform that allows sorting live cells from minimally viable cells and dead cells, while minimizing the risk of damage to the live cells during the sorting process. This process does not require that properties of the cell be known a priori, and allows for greater flexibility of sorting patterns. This platform is high-throughput and retrieves groups of sorted cells.