Learn more about UC TechAlerts – Subscribe to categories and get notified of new UC technologies

Browse Category: Research Tools > Nucleic Acids/DNA/RNA


[Search within category]

Lipid-Modified Oligonucleotides For Sample Barcoding in Droplet Microfluidics-Based Single-Cell RNA Sequencing

A new strategy for barcoding single living cells using lipid-modified oligonucleotides that can vastly enhance sample multiplexing in droplet microfluidics-based RNA sequencing

“SPARK (Separation of Phases-based Activity Reporter of Kinase)”_A Genetically-encoded Fluorescent Reporter Platform for Studying Cell Signaling in Living Cells

This novel class of genetically-encoded fluorescent reporters can be used as powerful tools to study protein-protein interactions (PPIs) in living cells. These bright, reversible reporters have a large dynamic range and fast kinetics, demonstrating significant advantages over traditional FRET-based fluorescent reporters.

EpiSort: A Novel Method Using Deep Bisulfite Sequencing to Determine Immune Cell Types in Solid Tissue Samples

EpiSort is a novel method of using DNA methylation patterns to determine the proportion of immune cell populations in solid tissue samples.

An Optimized Active Decapping Complex for Transcription Start Site Mapping

This invention describes an optimized, constitutive active decapping complex from S. pombe for efficient transcription start site (TSS) mapping.

Novel Method for Finding Low Abundance Sequences By Hybridization

This invention describes a novel method for enriching rare sequences in nucleic acid libraries.

PCR-Activated Sorting (PAS)

This invention identifies a novel method for nucleic acid sorting, whether free-floating or contained in structures (eg. cells and viruses) using droplet microfluidics and PCR.

SunTag: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

This research tool consists of a two-vector system that can recruit an amplified biological signal to intra-cellular targets of interest.

Novel Method of Using Modified and Optimized Bacterial-derived Genetic CRISPR System for Imaging, Regulating and Editing Mammalian Genomic Elements

This invention is a novel method using optimized small guide RNAs (sgRNAs) to enable dynamic imaging, editing and regulation of specific genomic elements in living mammalian cells via the CRISPR system.

Acat-2, A Second Mammalian Acyl Coa:Cholesterol Acyltransferase That Is Involved In Cholesterol Metabolism

Acyl-COA: cholesterol acyl transferases or ACAT is an enzyme that catalyzes the esterification of cholesterol to form cholesteryl ester. Minimally, ACAT-mediated formation of cholesteryl ester from cholesterol prevents the toxic accumulation of excess cholesterol in a cell and maintains a free diffusion gradient across the cell membrane, particularly in the small intestine. In addition, the assembly and secretion of Apolipoprotein-B containing lipoproteins in the liver and intestines is thought to be dependent on the ACAT-mediated formation of cholesteryl esters from cholesterol. In steroidogenic tissue such as the adrenal glands, ACAT activity produces cytosolic droplets loaded with cholesteryl esters from which they can be mobilized as cholesterol substrates for the generations of steroids. Furthermore, macrophages that accumulate cholesteryl ester in cytosolic lipid droplets as a result of ACAT activity appear foamy and are a characteristic early indicator of atherosclerotic lesions. Animal models that completely lack ACAT protein are viable, albeit with tissue-specific reductions in cholesteryl ester, suggesting that another ACAT enzyme is present in these animals.

  • Go to Page: