Programming Stem Cell Fate Using Designed Regulatory Proteins Without Genetic Modification
Tech ID: 21892 / UC Case 2008-311-0
BackgroundReprogramming human fibroblasts to become induced pluripotent stem cells (iPS cells) has opened another path to the derivation of stem cell lines with clinical and research potential. Unfortunately, these lines are not safe for human clinical use because, although useful in the short term, their genetic modifications may bear artifacts. Currently, derivation of iPS cells requires genetic modification using DNA transduction to introduce programming genes, causing permanent genetic modifications that can perturb cell fate in unpredictable ways.
Technology DescriptionUC San Diego investigators have uncovered a way of programming cell fate without genetic modifications. Designed Regulatory Proteins (DRPs) are artificial transcription factors that are fused to protein transduction domains. They can be designed to specifically regulate programming genes. Such DRPs that activate expression of key genes enable derivation of iPS cell colonies without causing any genetic modifications. Thus, this invention permits cell fate to be controlled in a precise and determinative way without making genetic modifications.
ApplicationsCommercial applications include reprogramming and programming cells to produce stem cells and differentiated cells for research and medicine. Safer, more predictable stem cell lines for clinical and research applications may be derived without genetic modification.
State Of DevelopmentThis technology is available for licensing. Worldwide rights are available.
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- Sera T, Uranga C. Rational design of artificial zinc-finger proteins using a nondegenerate recognition code table. Biochemistry. 2002 Jun 4;41(22):7074-81
|United States Of America||Published Application||20110318830||12/29/2011||2008-311|
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