Methods for Multiplex Digital PCR
Tech ID: 21714 / UC Case 2011-809-0
Researchers at the University of California, Irvine have developed methods to enable greater multiplexing abilities for digital polymerase chain reaction (PCR) so that up to 100 genetic targets may be analyzed. In the past multiplexing of digital PCR samples has been limited to only one probe per color. However multiple probes may be labeled by using combinatorial encoding of color, exploiting reaction rates of PCR cycles and modulating the intensity of Taqman and/or intercalating dyes therefore allowing a greater number of probes to be labeled.
100-plex digital PCR may be achieived. Currently, 7-plex PCR has been demonstrated in published literature.
PCR, Digital PCR, Multiplex, Microfluidics