Methods for Multiplex Digital PCR
Tech ID: 21714 / UC Case 2011-809-0
Patent Status
Patent Pending
Brief Description
Researchers at the University of California, Irvine have developed methods to enable greater multiplexing abilities for digital polymerase chain reaction (PCR) so that up to 100 genetic targets may be analyzed. In the past multiplexing of digital PCR samples has been limited to only one probe per color. However multiple probes may be labeled by using combinatorial encoding of color, exploiting reaction rates of PCR cycles and modulating the intensity of Taqman and/or intercalating dyes therefore allowing a greater number of probes to be labeled.
Advantages
100-plex digital PCR may be achieived. Currently, 7-plex PCR has been demonstrated in published literature.
Other Information
Categorized As
Related cases
2011-809-0
Keywords
PCR, Digital PCR, Multiplex, Microfluidics
Contact
Alvin Viray / aviray@uci.edu / tel: View Phone Number. Please reference Tech ID #21714.