Tech ID: 21418 / UC Case 2011-124-0
Surface patterning for single-cell culture is of great importance in studies dealing with cell shape and microenvironment effects on the motility, migration, proliferation, and differentiation of cells. These patterning techniques are key to effective cell printing needed for future medical advancements, such as 3D printing of artificial organs, tissue regeneration, and tissue engineering. Despite advances in surface patterning methods, important material surfaces such as glass cannot be easily patterned with established printing methods without prior surface modification.
Investigators at University of California at Berkeley have addressed this need by developing a single-cell patterning technique. This innovation is accomplished by coating the substrate surface with a hydrophobic film and then patterning the film surface. This surface patterning innovation for single-cell culture was achieved by combining plasma-assisted surface chemical modification, soft lithography, and protein-induced surface activation on glass. In a proof of concept study, the investigators have accomplished surfaces seeding with mesenchymal stem cells in serum medium, resulting in single-cell patterning.
In additional research, using a dry lithography method, hydrophilic surface patterns on polystyrene were directly applied to cell culture dishes without the requirement of clean-room facilities or chemicals that could be harmful to sensitive cells. The long-term stability of single-cell patterns on PS dish surfaces produced by the present method was accomplished in cell culture experiments with neuron stem cells (NSCs) and bovine aorta endothelial cells.
|United States Of America||Published Application||20130029421||01/31/2013||2011-124|
Additional Patent Pending
- aorta endothelial cells, neuron stem cells, and mesenchymal stem cells
- 2D & 3D tissue printing for artificial organ construction
- tissue regeneration
- long-term cell culture and differentiation
- easily patterns glass, polystyrene & other nonstandard substrates
- excellent biocompatibility; chemically stable
- no clean-room or harsh chemicals required
- wide range of pattern shapes and sizes
- Cheng, Qian
- Komvopoulos, Kyriakos