Electrophysiological Cell Cytometry And Sorting
Tech ID: 21199 / UC Case 2011-065-0
Brief DescriptionAn interdisciplinary team of researchers has developed a microfluidics platform that uses electrophysiological signatures to sort living cells by their functionality. Because this method does not use exogenous labels, the purified cells are compatible with clinical translation. This includes a range of electrically-excitable cells, such as cardiomyocytes, neurons, and smooth muscle cells.
This technology represents a new approach to cell sorting that does not rely on physical markers or protein expression profile. The platform is aimed at generating highly-pure populations of electrically active cells from heterogeneous stem cell progeny which is particular useful for regenerative medicine and tissue engineering, with additional applications in drug screening and basic research.
Stage of Research
The inventors have built a prototype device and used it to purify induced pluripotent stem cell (iPSC) cardiomyocytes from undifferentiated iPSC clusters.
- no extra labeling step
- technique can be used for cells without identified biomarkers
- purified cells are compatible with clinical use
- No genetic modification
-Specific - low false positive rate from distinct electrophysiological signals (could reduce the rate of teratoma formation from undifferentiated cells)
- Functional assessment - dynamic signals from living cells
- Abilez, Oscar J.
- Lee, Luke P.
- Myers, Frank B.
- Zarins, Christopher
ADDITIONAL TECHNOLOGIES BY THESE INVENTORS
- Microfluidic Sample Preparation And Impedimetric Detection Of Small Molecules
- Monodisperse Silk Emulsions And Microspheres
- Low Cost Portable Diagnostic Biomolecular Detection Platform
- Resettable Microfluidic device- Microfluidic Ping Pong (MPP)
- A Porous Microfluidic Spinneret
- Biologically Inspired Self-Activated Building Envelope Regulation (Saber)
- Solar Optics-Based Active Panels (Soap) For Greywater Reuse And Integrated Thermal (Grit) Building Control
- Integrated Microfluidic Universal Sample Preparation And Preconcentration (Usp) Module
- Surface-enhanced Optical Upconversion Luminescence (SOUL) for Single miRNA Detection
- Nanophotonic Graphene Transistor
- Modular Aptazyme-Mediated Signal Transduction Coupled With Chemical Amplification In A Semi-Quantitative, Colorimetric Diagnostic Assay
- Stencil Patterning Method For Generating Highly Uniform Stem Cell Colonies
- Photonic Gene Circuits
- Low Cost Integrated Molecular Diagnostic Systems