Synthetic Surfaces For Defined Human Cell Culture
Tech ID: 21056 / UC Case 2011-019-0
Brief Description
Researchers at UC Berkeley have developed a synthetic polymer interface for the long-term self-renewal of human embryonic stem cells (hESCs) in defined media. Current culture systems for hESCs require the use of isolated animal derived extracellular matrix proteins or mouse embryonic feeder cells. The proposed use of a completely synthetic cell culture substrate avoids the problems associated with the variability of and the exposure to animal products. The hydrogel network coating is comprised of aminopropylmethacrylamide (APMAAm) monomer and N,N-methylenebis(acrylamide) (bis) crosslinker that was grafted to standard tissue culture polystyrene (TCPS) dishes via photoinitiated addition polymerization. Results for hESC proliferation and pluripotency markers were both qualitatively and quantitatively similar to cells cultured on MatrigelÔ -coated substrates.
Patent Status
| Country | Type | Number | Dated | Case |
| Patent Cooperation Treaty | Published Application | WO2012/106459 | 08/09/2012 | 2011-019 |
Additional Patent Pending
Advantages
Allows for the long-term self-renewal of hESCs in completely defined conditions.
Does not require attachment of peptides or proteins to promote cell attachment.
Is readibly scalable to clinical-scale cell expansion.
Free of complex, undefined culture materials.
Low cost.
Inventors
- Healy, Kevin E.
- Irwin, Beth
Other Information
Categorized As
Related cases
2011-019-0
Keywords
stem cell, culture, embryonic, hydrogel, biointerface, self-renewal
Contact
Irvin J. Mettler / imettler@berkeley.edu / tel: View Phone Number. Please reference Tech ID #21056.
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