Hepatitis C Virus Genotype 1b Cell Cultures
Tech ID: 21002 / UC Case 2010-173-0
For producing RNA viruses, applications such as drug screening or basic research require immortalized tissue cultures that enable efficient production of fully infectious viruses of a genotype of clinical interest and are easy to use. In the case of hepatitis C virus (HCV), however, existing tissue culture systems yield incompletely replicated viruses (which do not infect cells in vitro), work only transiently, or are not robust enough and otherwise yield viruses with less prevalent HCV genotypes such as genotype 2. Thus, there is a need for a continuous or semi-continuous HCV production system that overcomes these limitations with the generation of a fully infectious HCV of genotype 1.
Researchers at the University of California, Merced (UCM) have stably transfected immortalized human cells with a CG1b construct that can release infectious HCV of genotype 1b into the cell culture media. The UCM researchers demonstrated the infectivity of this media using Huh7 and Huh7.5 cells (figure 1).
|FIGURE 1—Infectivity of the CG1b media of this invention containing HCV genotype 1b and of a JFH1 media containing HCV genotype 2a.|
The UCM tissue culture system may find widespread use in numerous research and drug discovery applications where efficient and continuous production of completely replicated, fully infectious HCV is required.
The UCM tissue culture system, along with the JFH1 system, are the only continuous tissue culture systems that can efficiently generate HCV that can infect Huh and other cells in vitro. Unlike JFH1, this invention can replicate HCV genotype 1b, which is more prevalent and of greater clinical interest than the HCV genotype 2 produced by JFH1.
|United States Of America||Published Application||20110124086||05/26/2011||2010-173|
Additional Patent Pending
- Choi, Jinah
- Liang, T. Jake
2010-173-0, 2011-048-1, 2011-128-1
hepatitis C virus, tissue culture system
ADDITIONAL TECHNOLOGIES BY THESE INVENTORS
PEOPLE WHO VIEWED THIS ALSO VIEWED THESE TECHNOLOGIES BY OTHER INVENTORS
- A Novel Biomarker for Irritable Bowel Syndrome and Other Stress Disorders
- Device to Characterize Gas Transport Properties Of Cell-Free Oxygen Carriers And Red Blood Cells
- Treatment of Liver Fibrosis by Induction of Stellate Cell Apoptosis
- SMALL MOLECULE INHIBITORS OF CALCIUM-ACTIVATED CHLORIDE CHANNELS TO TREAT SECRETORY DIARRHEA
- Use of Catestatin to Mobilize Fat from Adipose Tissue by Regulating Adrenergic and Leptin Signaling