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Cas12a System For Combinatorial Transcriptional Repression In Eukaryotic Cells
Brief description not available
High-Throughput Discovery Of Bipartite Or Tripartite Crispr-Based Epigenetic Editors
Currently, conventional methods of epigenome editor discovery require time and labor-intensive construct development, which is typically performed in low-throughput arrayed formats. The platform bypasses current time/labor constraints (and without reliance on construct barcodes) to facilitate the identification of an optimal gene modulator in a single experiment.
CRISPR-Cas Inhibiting Polypeptides
ENABLING GENETIC ANALYSIS OF DIVERSE BACTERIA WITH MOBILE-CRISPRi
Researchers at UCSF, funded in part by the Chan Zuckerberg Biohub, have generated a modular and effective CRISPRi system for the genetic dissection of non-model bacteria.
Genome-Wide Interaction Screens In Primary Human Cells For Target Discovery And Drug Validation.
4D-seq: Single Cell RNA-sequencing with in situ Spatiotemporal Information
To develop a novel imaging-based single cell RNA-sequencing (scRNA-Seq) platform that allows capturing of spatiotemporal information and cellular behavior of the sequenced cells within tissue.
Lipid-Modified Oligonucleotides For Sample Barcoding in Droplet Microfluidics-Based Single-Cell RNA Sequencing
A new strategy for barcoding single living cells using lipid-modified oligonucleotides that can vastly enhance sample multiplexing in droplet microfluidics-based RNA sequencing
“SPARK (Separation of Phases-based Activity Reporter of Kinase)”_A Genetically-encoded Fluorescent Reporter Platform for Studying Cell Signaling in Living Cells
This novel class of genetically-encoded fluorescent reporters can be used as powerful tools to study protein-protein interactions (PPIs) in living cells. These bright, reversible reporters have a large dynamic range and fast kinetics, demonstrating significant advantages over traditional FRET-based fluorescent reporters.
An Optimized Active Decapping Complex for Transcription Start Site Mapping
This invention describes an optimized, constitutive active decapping complex from S. pombe for efficient transcription start site (TSS) mapping.
Novel Method for Finding Low Abundance Sequences By Hybridization
This invention describes a novel method for enriching rare sequences in nucleic acid libraries.
Novel Method of Using Modified and Optimized Bacterial-derived Genetic CRISPR System for Imaging, Regulating and Editing Mammalian Genomic Elements
This invention is a novel method using optimized small guide RNAs (sgRNAs) to enable dynamic imaging, editing and regulation of specific genomic elements in living mammalian cells via the CRISPR system.